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Image Search Results
Journal:
Article Title: Novel transcripts of Nox1 are regulated by alternative promoters and expressed under phenotypic modulation of vascular smooth muscle cells
doi: 10.1042/BJ20060300
Figure Lengend Snippet: (A) Schematic diagram of the promoter-luciferase fusion plasmids is shown on the left, where the 5′/3′ ends of the construct relative to the transcription initiation site are indicated. Each construct was transiently transfected into P53LMACO1 (closed bar) or MCE301 cells (opened bar). The β-galactosidase-expression vector was co-transfected as an internal control. The relative luciferase activity was denoted as the ratio to the activity of the pGL3-basic vector. Bars represent means ±SE of at least three experiments.
Article Snippet: The 5′-flanking regions of the corresponding exons for the identified transcripts were isolated from a mouse genomic library (Lambda FIXII Mouse 129/SVJ, Stratagene, La Jolla, CA, U.S.A.), and cloned into a
Techniques: Luciferase, Construct, Transfection, Expressing, Plasmid Preparation, Activity Assay